CD8 Rabbit Polyclonal Antibody
cat.: 0108-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse CD8 aa 21-70 / 247. |
| Positive control: | Mouse thymus tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:500 1:50 |
| Uniprot #: | SwissProt: P01731 Mouse |
| Alternative names: | CD8 CD8 antigen, alpha polypeptide (p32) CD8a CD8a antigen CD8a molecule CD8A_HUMAN Leu2 Leu2 T lymphocyte antigen MAL OKT8 T cell antigen p32 T cell antigen Leu2 T cell co receptor T-cell surface glycoprotein CD8 alpha chain T-lymphocyte differentiation antigen T8/Leu-2 T8 T cell antigen |
Images
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Fig1:
Western blot analysis of CD8 on Mouse thymus tissue lysates with Rabbit anti-CD8 antibody (0108-7) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 27 kDa Observed band size: 35 kDa Exposure time: 1 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0108-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: C57BL/6 mouse spleen Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 0108-7, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.