Anti-Cytokeratin 18 antibody
cat.: 0407-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Cytokeratin 18 aa 374-423 / 423.
Positive control: Mouse liver tissue, 293T, A549, SW480, mouse kidney tissue.
Subcellular location: Cytoplasm, perinuclear region.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:25,000-50,000
1:200
1:100
Uniprot #: SwissProt: P05783 Human | P05784 Mouse | Q5BJY9 Rat
Alternative names: Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 Cell proliferation-inducing gene 46 protein CK 18 CK-18 CK18 CYK 18 CYK18 Cytokeratin 18 Cytokeratin endo B Cytokeratin-18 K 18 K18 K1C18_HUMAN KA18 Keratin 18 Keratin 18, type I Keratin D keratin, type I cytoskeletal 18 Keratin-18 Krt18
Images
0407-1_1.jpg Fig1: Western blot analysis of Cytokeratin 18 on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
0407-1_2.jpg Fig2: ICC staining Cytokeratin 18 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-1) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
0407-1_3.jpg Fig3: ICC staining Cytokeratin 18 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-1) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
0407-1_4.jpg Fig4: ICC staining Cytokeratin 18 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-1) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
0407-1_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-1) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
0407-1_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytokeratin 18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-1) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.