Synaptophysin Rabbit Polyclonal Antibody
cat.: 0407-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide (KLH-coupled) within human Synaptophysin aa 220-280. |
| Positive control: | Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44. |
| Subcellular location: | Cytoplasmic vesicle, Membrane, Synapse, Synaptosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2000-1:10,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P08247 Human | Q62277 Mouse | P07825 Rat |
| Alternative names: | Major synaptic vesicle protein p38 MRX96 MRXSYP Syn p38 Synaptophysin Syp SYPH SYPH_HUMAN SypI |
Images
|
Fig1:
Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (0407-2) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0407-2, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 34 kDa Observed band size: 37 kDa |
|
Fig2: ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.