Cyclin D1 Rabbit Polyclonal Antibody
cat.: 0407-27
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 34 kDa
Isotype: IgG
Immunogen: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of cyclin D1.
Positive control: MCF-7, A549, HepG2, N2A.
Subcellular location: Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:5,000
1:200
1:50-1:100
Uniprot #: SwissProt: P24385 Human | P25322 Mouse | P39948 Rat
Alternative names: AI327039 B cell CLL/lymphoma 1 B cell leukemia 1 B cell lymphoma 1 protein B-cell lymphoma 1 protein BCL 1 BCL-1 BCL-1 oncogene BCL1 BCL1 oncogene ccnd1 CCND1/FSTL3 fusion gene, included CCND1/IGHG1 fusion gene, included CCND1/IGLC1 fusion gene, included CCND1/PTH fusion gene, included CCND1_HUMAN cD1 Cyl 1 D11S287E G1/S specific cyclin D1 G1/S-specific cyclin-D1 Parathyroid adenomatosis 1 PRAD1 PRAD1 oncogene U21B31
Images
0407-27_1.jpg Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
0407-27_2.jpg Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
0407-27_3.jpg Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
0407-27_4.jpg Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-27) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
0407-27_5.jpg Fig5: Flow cytometric analysis of Cyclin D1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Cyclin D1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.