alpha Actin (cardiac actin) Rabbit Polyclonal Antibody
cat.: 0407-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human alpha-Actin (cardiac actin) aa 1-50 / 377.
Positive control: Rat heart tissue lysates, mouse heart tissue lysates, hybrid fish (crucian-carp) heart tissue lysates, rat heart tissue, human heart tissue, mouse heart tissue.
Subcellular location: Cytoplasma , etc.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:400
Uniprot #: SwissProt: P68032 Human | P68033 Mouse
Entrez Gene: 29275 Rat
Alternative names: ACTC ACTC_HUMAN ACTC1 Actin alpha cardiac muscle 1 Actin alpha cardiac muscle 1 Alpha-cardiac actin ASD5 CMD1R CMH11 LVNC4
Images
0407-3_1.jpg Fig1: Western blot analysis of alpha Actin (cardiac actin) on different lysates with Rabbit anti-alpha Actin (cardiac actin) antibody (0407-3) at 1/500 dilution.

Lane 1: Rat heart tissue lysate
Lane 2: Mouse heart tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-3) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
0407-3_2.jpg Fig2: Western blot analysis of Alpha-cardiac actin on hybrid fish (crucian-carp) heart tissue lysate using anti-Alpha-cardiac actin antibody at 1/500 dilution.
0407-3_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-alpha Actin (cardiac actin) antibody (0407-3) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0407-3_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-alpha Actin (cardiac actin) antibody (0407-3) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0407-3_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-alpha Actin (cardiac actin) antibody (0407-3) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.