Alpha-cardiac actin Rabbit Polyclonal Antibody
cat.: 0407-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human alpha-Actin (cardiac actin) aa 1-50 / 377. |
| Positive control: | Rat heart tissue lysate, Mouse heart tissue lysate, C2C12 cell lysate,hybrid fish (crucian-carp) heart tissue lysates, human heart tissue, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Cytoplasma , etc. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:400 |
| Uniprot #: | SwissProt: P68032 Human | P68033 Mouse Entrez Gene: 29275 Rat |
| Alternative names: | ACTC ACTC_HUMAN ACTC1 Actin alpha cardiac muscle 1 Actin alpha cardiac muscle 1 Alpha-cardiac actin ASD5 CMD1R CMH11 LVNC4 |
Images
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Fig1:
Western blot analysis of Alpha-cardiac actin on different lysates with Rabbit anti-Alpha-cardiac actin antibody (0407-3) at 1/1,000 dilution. Lane 1: C2C12 cell lysate(20 µg/Lane) Lane 2: Mouse heart tissue lysate(40 µg/Lane) Lane 3: Rat heart tissue lysate(40 µg/Lane) Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0407-3, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
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Fig2: Western blot analysis of Alpha-cardiac actin on hybrid fish (crucian-carp) heart tissue lysate using anti-Alpha-cardiac actin antibody at 1/500 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Alpha-cardiac actin antibody (0407-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Alpha-cardiac actin antibody (0407-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Alpha-cardiac actin antibody (0407-3) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-3) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.