14-3-3 alpha+beta Rabbit Polyclonal Antibody
cat.: 0407-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse 14-3-3 beta / alpha aa 197-246 / 246. |
| Positive control: | Hela cell lysate, human brain tissue lysate, mouse brain tissue lysate, N2A. |
| Subcellular location: | Cytoplasm, Melanosome. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:200 |
| Uniprot #: | SwissProt: P31946 Human | Q9CQV8 Mouse |
| Alternative names: | 14 3 3 alpha 14 3 3 protein beta/alpha 14-3-3 protein beta/alpha 1433B_HUMAN Brain protein 14 3 3 beta isoform GW128 HS 1 KCIP-1 KCIP1 N-terminally processed Protein 1054 Protein kinase C inhibitor protein 1 YWHAA YWHAB |
Images
|
Fig1:
Western blot analysis of 14-3-3 alpha+beta on different lysates with Rabbit anti-14-3-3 alpha+beta antibody (0407-5) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-14-3-3 alpha+beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody. Lane 1: Hela cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse brain tissue lysate Predicted band size: 28 kDa Observed band size: 28 kDa |
|
Fig3: ICC staining 14-3-3 b/a in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.