Caveolin-1 Rabbit Polyclonal Antibody
cat.: 0407-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Caveolin-1 aa 128-178. |
| Positive control: | Mouse liver tissue lysates, human kidney tissue. |
| Subcellular location: | Golgi apparatus membrane, Cell membrane, Membrane, caveola, Membrane raft, Golgi apparatus, trans-Golgi network. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q03135 Human | P49817 Mouse | P41350 Rat |
| Alternative names: | BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Caveolin-1 antibody (0407-6) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-6) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of Caveolin-1 on different lysates with Rabbit anti-Caveolin-1 antibody (0407-6) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat lung tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0407-6, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 21 kDa Observed band size: 25 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.