Caveolin-1 Rabbit Polyclonal Antibody
cat.: 0407-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Caveolin-1 aa 128-178. |
| Positive control: | Mouse liver tissue lysates, human kidney tissue. |
| Subcellular location: | Golgi apparatus membrane, Cell membrane, Membrane, caveola, Membrane raft, Golgi apparatus, trans-Golgi network. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: Q03135 Human | P49817 Mouse | P41350 Rat |
| Alternative names: | BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21 |
Images
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Fig1: Western blot analysis on Mouse liver tissue lysate using anti-Caveolin-1 polyclonal antibody |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Caveolin-1 antibody (0407-6) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-6) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.