DPPA2 Rabbit Polyclonal Antibody
cat.: 0731-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse DPPA2 aa 1-50 / 301. |
| Positive control: | F9, human colon tissue, human testis tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:500 1:2,000 |
| Uniprot #: | SwissProt: Q9CWH0 Mouse |
| Alternative names: | cancer/testis antigen 100 CT100 Developmental Pluripotency Associated 2 developmental pluripotency-associated gene 2 developmental pluripotency-associated protein 2 ECAT15-2 embryonic stem cell (ESC) associated transcript 15-2 PESCRG1 Pluripotent embryonic stem cell related protein 1 Pluripotent embryonic stem cell-related gene 1 Pluripotent embryonic stem cell-related gene 1 protein |
Images
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Fig1: Western blot analysis on F9 using anti-DPPA2 polyclonal antibody |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-DPPA2 antibody (0731-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0731-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DPPA2 antibody (0731-4) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0731-4) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.