Estrogen Receptor alpha Rabbit Polyclonal Antibody
cat.: 0803-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C terminal human estrogen receptor. |
| Positive control: | MCF-7, human breast tissue, SK-Br-3. |
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:100 1:50 |
| Uniprot #: | SwissProt: P03372 Human |
| Alternative names: | DKFZp686N23123 ER alpha ER ER-alpha Era ESR ESR1 ESR1_HUMAN ESRA Estradiol receptor Estrogen nuclear receptor alpha Estrogen receptor 1 Estrogen receptor alpha 3*,4,5,6,7*/822 isoform Estrogen receptor alpha Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform Estrogen receptor alpha delta 4 +49 isoform Estrogen receptor alpha delta 4*,5,6,7*/654 isoform Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform Estrogen receptor alpha E1 E2 1 2 Estrogen receptor alpha E1 N2 E2 1 2 Estrogen receptor ESTRR NR3A1 Nuclear receptor subfamily 3 group A member 1 |
Images
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Fig1: Western blot analysis on MCF-7 using anti-ESR1 polyclonal antibody |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0803-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SK-Br-3 cells labeling Estrogen Receptor alpha with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Estrogen Receptor alpha antibody (0803-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.