| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse SCA-1 aa 51-94. |
| Positive control: | Mouse kidney tissue lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P05533 Mouse |
| Alternative names: | Ly-6A.2/Ly-6E. Ly-6A.2/Ly-6E.1 Ly6a LY6A_MOUSE Ly6al Lymphocyte antigen 6A-2/6E-1 Lymphocyte antigen Ly 6A.2/Ly 6E.1 SCA-1 Stem cell antigen 1 T cell activating protein T-cell-activating protein TAP |
|
Fig1:
Western blot analysis of SCA-1 on different lysates with Rabbit anti-SCA-1 antibody (0804-10) at 1/,000 dilution. Lane 1: Mouse lung tissue lysate Exposure time: 10s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0804-10, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 14 kDa Observed band size: 14 kDa |