SCA-1 Rabbit Polyclonal Antibody
cat.: 0804-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse SCA-1 aa 51-94. |
| Positive control: | Mouse kidney tissue lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P05533 Mouse |
| Alternative names: | Ly-6A.2/Ly-6E. Ly-6A.2/Ly-6E.1 Ly6a LY6A_MOUSE Ly6al Lymphocyte antigen 6A-2/6E-1 Lymphocyte antigen Ly 6A.2/Ly 6E.1 SCA-1 Stem cell antigen 1 T cell activating protein T-cell-activating protein TAP |
Images
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Fig1:
Western blot analysis of SCA-1 on different lysates with Rabbit anti-SCA-1 antibody (0804-10) at 1/500 dilution. Lane 1: Mouse kidney tissue lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.