SCA-1 Rabbit Polyclonal Antibody
cat.: 0804-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Mouse SCA-1 aa 51-94.
Positive control: Mouse kidney tissue lysate, NIH/3T3 cell lysate.
Subcellular location: Cell membrane
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P05533 Mouse
Alternative names: Ly-6A.2/Ly-6E. Ly-6A.2/Ly-6E.1 Ly6a LY6A_MOUSE Ly6al Lymphocyte antigen 6A-2/6E-1 Lymphocyte antigen Ly 6A.2/Ly 6E.1 SCA-1 Stem cell antigen 1 T cell activating protein T-cell-activating protein TAP
Images
0804-10_1.jpg Fig1: Western blot analysis of SCA-1 on different lysates with Rabbit anti-SCA-1 antibody (0804-10) at 1/,000 dilution.

Lane 1: Mouse lung tissue lysate

Exposure time: 10s seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0804-10, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 14 kDa
Observed band size: 14 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.