Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, ICC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Peptide affinity purified. |
Molecular weight: | 125kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N-terminal Human TIE-1. |
Positive control: | K562, HepG2, SW620. |
Subcellular location: | Cell membrane. Membrane. |
Recommended Dilutions:
WB ICC |
1:1,000-1:5,000 1:50-1:200 |
Uniprot #: | SwissProt: P35590 Human |
Alternative names: | JKT 14 antibody JTK14 antibody TIE antibody Tie1 antibody TIE1_HUMAN antibody Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody Tyrosine protein kinase receptor Tie 1 antibody Tyrosine-protein kinase receptor Tie-1 antibody |
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Fig1: Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution. |
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Fig2: ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |