TIE-1 Rabbit Polyclonal Antibody
cat.: 0804-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 125 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TIE-1 aa 23-72 / 1,138. |
| Positive control: | HUVEC cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HepG2, SW620. |
| Subcellular location: | Cell membrane. Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P35590 Human | Q06806 Mouse Entrez Gene: 89806 Rat |
| Alternative names: | JKT 14 JTK14 TIE Tie1 TIE1_HUMAN Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 Tyrosine protein kinase receptor Tie 1 Tyrosine-protein kinase receptor Tie-1 |
Images
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Fig1:
Western blot analysis of TIE-1 on different lysates with Rabbit anti-TIE-1 antibody (0804-11) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: K-562 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 125 kDa Observed band size: 125 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-11) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.