CD24 Rabbit Polyclonal Antibody
cat.: 0804-4
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 8 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD24 aa 31-80 / 80.
Positive control: Human spleen tissue lysates, Hela, SW480, MCF-7.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P25063 Human
Alternative names: CD 24 CD24 CD24 antigen (small cell lung carcinoma cluster 4 antigen) CD24 antigen CD24 molecule CD24_HUMAN CD24A FLJ22950 FLJ43543 GPI linked surface mucin Heat stable antigen HSA MGC75043 Nectadrin Signal transducer CD24 Small cell lung carcinoma cluster 4 antigen
Images
0804-4_1.jpg Fig1: Western blot analysis of CD24 on human spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0804-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
0804-4_2.jpg Fig2: ICC staining of CD24 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (0804-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.
0804-4_3.jpg Fig3: ICC staining of CD24 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (0804-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
0804-4_4.jpg Fig4: Flow cytometric analysis of CD24 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (0804-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.