CD133 Rabbit Polyclonal Antibody
cat.: 0804-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 97 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD133 aa 800-865. |
| Positive control: | Human stomach tissue lysates, PC-12 cell lysates, F9. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O43490 Human | O54990 Mouse |
| Alternative names: | AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4 |
Images
|
Fig1: Western blot analysis of CD133 on human stomach tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CD133 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Flow cytometric analysis of CD133 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (0804-5, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.