| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse GM-CSF-R-alpha (CD116) aa 11-60 / 388. |
| Positive control: | F9 cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:100-1:200 1:10-1:100 |
| Uniprot #: | SwissProt: Q00941 Mouse |
| Alternative names: | CD_antigen=CD116 CD116 CD116 antigen CDw116 Colony stimulating factor 2 receptor alpha chain Colony stimulating factor 2 receptor alpha low affinity Colony stimulating factor 2 receptor alpha subunit CSF 2R CSF2R CSF2R_HUMAN CSF2RA CSF2RAX CSF2RAY CSF2RX CSF2RY GM CSF R alpha GM CSF receptor alpha subunit GM-CSF-R-alpha GMCSFR GMCSFR-alpha GMR-alpha Granulocyte macrophage colony stimulating factor receptor alpha chain Granulocyte-macrophage colony-stimulating factor receptor subunit alpha SMDP4 |
|
Fig1:
Western blot analysis of GM-CSF-R-alpha (CD116) on mouse skin lysates with Rabbit anti-GM-CSF-R-alpha (CD116) antibody (0804-8) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0804-8, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 70 kDa |