Beta III Tubulin Rabbit Polyclonal Antibody
cat.: 0805-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human Neuron-specific class Ⅲ β-tubulin. |
| Positive control: | Hela cell lysate, HepG2, NIH/3T3, rat brain tissue, rat kidney tissue, human colon cancer tissue. |
| Subcellular location: | Cytoplasm. Cytoskeleton. Microtubule, Cell projection. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000-1:10,000 1:50-1:200 1:100-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: Q13509 Human | Q9ERD7 Mouse | Q4QRB4 Rat |
| Alternative names: | beta 3 tubulin beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III |
Images
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Fig1: Western blot analysis of Beta III Tubulin on Hela cell lysate using anti-Beta III Tubulin antibody at 1/10,000 dilution. |
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Fig2: ICC staining Beta III Tubulin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Beta III Tubulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Beta III Tubulin antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Beta III Tubulin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Beta III Tubulin antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with Beta III Tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.