0805-4

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, ICC, IHC-P, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1 mg/mL.

Purification:

Immunogen affinity purified

Molecular weight:

85kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within Human CD10 aa 200-254.

Positive control:

Human kidney tissue, mouse kidney tissue, rat kidney tissue, Jurkat, HepG2.

Subcellular location:

Cell membrane. Membrane.

Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100

Uniprot #:

P08473(Human) Q61391(Mouse) P07861(Rat)

Alternative names:

Atriopeptidase antibody CALLA antibody CD10 antibody CD10 antigen antibody Common acute lymphocytic leukemia antigen antibody DKFZp686O16152 antibody EC 3.4.24.11 antibody Enkephalinase antibody EPN antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody neprilysin-390 antibody neprilysin-411 antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody Neutral endopeptidase, membrane-associated antibody SFE antibody Skin fibroblast elastase antibody

	Fig1: Western blot analysis of CD10 on mouse kidney lysate using anti-CD10 antibody at 1/500 dilution.
	Fig2: ICC staining CD10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
	Fig6: Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.