0805-4

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 86 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within Human CD10 aa 205-254 / 750.

Positive control:

LNCaP cell lysate, Ramos cell lysate, Raji cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Subcellular location:

Cell membrane.

Recommended Dilutions:
WB
IHC-P

1:2,000
1:50-1:200

Uniprot #:

SwissProt: P08473 Human | Q61391 Mouse | P07861 Rat

Alternative names:

Atriopeptidase CALLA CD10 CD10 antigen Common acute lymphocytic leukemia antigen DKFZp686O16152 EC 3.4.24.11 Enkephalinase EPN Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) Membrane metallo endopeptidase Membrane metallo endopeptidase variant 1 Membrane metallo endopeptidase variant 2 Membrane metalloendopeptidase Membrane metalloendopeptidase neutral endopeptidase enkephalinase Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 Membrane metalloendopeptidase variant 1 Membrane metalloendopeptidase variant 2 MGC126681 MGC126707 MME NEP NEP_HUMAN Neprilysin neprilysin-390 neprilysin-411 Neutral endopeptidase 24.11 Neutral endopeptidase Neutral endopeptidase, membrane-associated SFE Skin fibroblast elastase

	Fig1: Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (0805-4) at 1/2,000 dilution. Lane 1: LNCaP cell lysate Lane 2: Ramos cell lysate Lane 3: HT-29 cell lysate (negative) Lane 4: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.