CD62E Rabbit Polyclonal Antibody
cat.: 0805-6
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 67 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD62E aa 26-75 / 610.
Positive control: Hela, MCF-7 cell lysate, Raji cell lysate, HepG2, Jurkat, MCF-7.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC

1:500
1:200
1:50-1:100
Uniprot #: SwissProt: P16581 Human
Alternative names: CD26E CD62 antigen-like family member E CD62E E selectin E-selectin ELAM ELAM-1 ELAM1 Endothelial adhesion molecule 1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN Sele Selectin E
Images
0805-6_1.jpg Fig1: Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Raji cell lysate
0805-6_2.jpg Fig2: ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-6_3.jpg Fig3: ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-6_4.jpg Fig4: ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-6_5.jpg Fig5: Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.