CD62E Rabbit Polyclonal Antibody
cat.: 0805-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD62E aa 26-75 / 610. |
| Positive control: | Hela, MCF-7 cell lysate, Raji cell lysate, HepG2, Jurkat, MCF-7. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P16581 Human |
| Alternative names: | CD26E CD62 antigen-like family member E CD62E E selectin E-selectin ELAM ELAM-1 ELAM1 Endothelial adhesion molecule 1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN Sele Selectin E |
Images
|
Fig1:
Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Raji cell lysate |
|
Fig2: ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5:
Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.