CD3 zeta Rabbit Polyclonal Antibody
cat.: 0805-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human CD3 zeta. |
| Positive control: | Jurkat cell lysate, A549, Hela, HT-29, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P20963 Human |
| Alternative names: | 4930549J05Rik A430104F18Rik AW552088 CD247 CD247 antigen Cd247 molecule Cd3 CD3 antigen, zeta polypeptide, isoform CRA_b CD3 antigen, zeta subunit CD3-eta CD3H CD3Q Cd3z CD3Z antigen zeta polypeptide (TiT3 complex) CD3Z_HUMAN CD3zeta CD3zeta chain MGC140430 T cell receptor T3 zeta chain T cell surface glycoprotein CD3 zeta chain T-cell antigen receptor complex, zeta subunit of CD3 T-cell receptor T3 zeta chain T-cell surface glycoprotein CD3 zeta chain T3z TCR zeta chain TCRk TCRZ TCRzeta |
Images
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Fig1: Western blot analysis of CD3 zeta on Jurkat cell lysate using anti-CD3 zeta antibody at 1/1,000 dilution. |
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Fig2: ICC staining CD3 zeta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD3 zeta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CD3 zeta in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of Jurkat cells with CD3 zeta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.