CD3 zeta Rabbit Polyclonal Antibody
cat.: 0805-7
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 19kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human CD3 zeta.
Positive control: Jurkat, A549, Hela, HT-29.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  ICC
  FC

1:1,000
1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: P20963 Human
Alternative names: 4930549J05Rik A430104F18Rik AW552088 CD247 CD247 antigen Cd247 molecule Cd3 CD3 antigen, zeta polypeptide, isoform CRA_b CD3 antigen, zeta subunit CD3-eta CD3H CD3Q Cd3z CD3Z antigen zeta polypeptide (TiT3 complex) CD3Z_HUMAN CD3zeta CD3zeta chain MGC140430 T cell receptor T3 zeta chain T cell surface glycoprotein CD3 zeta chain T-cell antigen receptor complex, zeta subunit of CD3 T-cell receptor T3 zeta chain T-cell surface glycoprotein CD3 zeta chain T3z TCR zeta chain TCRk TCRZ TCRzeta
Images
0805-7_1.jpg Fig1: Western blot analysis of CD3 zeta on Jurkat cell lysate using anti-CD3 zeta antibody at 1/1,000 dilution.
0805-7_2.jpg Fig2: ICC staining CD3 zeta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-7_3.jpg Fig3: ICC staining CD3 zeta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-7_4.jpg Fig4: ICC staining CD3 zeta in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0805-7_5.jpg Fig5: Flow cytometric analysis of Jurkat cells with CD3 zeta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.