| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Alkaline Phosphatase(PLAP-1 / PLAP-like) aa 377-426 / 535. |
| Positive control: | Hela cell lysate. |
| Subcellular location: | Cell membrane, Lipid-anchor, GPI-anchor. |
| Recommended Dilutions:
WB |
1:5000 |
| Uniprot #: | SwissProt: P05187 Human |
| Alternative names: | Alkaline phosphatase Alkaline phosphatase placental Alkaline phosphatase placental type Alkaline phosphatase Regan isozyme ALP Alp1 ALPP FLJ61142 Germ-cell alkaline phosphatase nagao isozyme OTTHUMP00000164354 PALP Placental alkaline phosphatase 1 placental heat-stable alkaline phosphatase placental type PLAP PLAP-1 PLAP1 PPB1_HUMAN |
|
Fig1:
Western blot analysis of Alkaline Phosphatase(PLAP-1/PLAP-like) on different lysates with Rabbit anti-Alkaline Phosphatase(PLAP-1/PLAP-like) antibody (0806-4) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: SiHa (Human cervical cancersSquamous cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0806-4, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58 kDa Observed band size: 58 kDa |