CD146 Rabbit Polyclonal Antibody
cat.: 0806-5
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD146 aa 597-646 / 646. |
| Positive control: | HUVEC cell lysate, Mouse white adipose tissue lysate, Rat white adipose tissue lysate, Rat brown adipose tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P43121 Human | Q8R2Y2 Mouse | Q9EPF2 Rat |
| Alternative names: | A32 antigen CD 146 CD146 CD146 antigen Cell surface glycoprotein MUC18 Cell surface glycoprotein P1H12 Gicerin Mcam Melanoma adhesion molecule Melanoma associated antigen A32 Melanoma associated antigen MUC18 Melanoma associated glycoprotein MUC18 Melanoma cell adhesion molecule Melanoma-associated antigen A32 Melanoma-associated antigen MUC18 MelCAM MUC 18 MUC18 MUC18_HUMAN S endo 1 S endo 1 endothelial associated antigen S-endo 1 endothelial-associated antigen |
Images
|
Fig1:
Western blot analysis of CD146 on different lysates with Rabbit anti-CD146 antibody (0806-5) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: Mouse white adipose tissue lysate Lane 4: Rat white adipose tissue lysate Lane 5: Rat brown adipose tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 120 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0806-5) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
CD146 was immunoprecipitated from 0.2 mg HUVEC cell lysate with 0806-5 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 0806-5 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HUVEC cell lysate (input) Lane 2: 0806-5 IP in HUVEC cell lysate Lane 3: Rabbit IgG instead of 0806-5 in HUVEC cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 13 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.