Glycophorin A Rabbit Polyclonal Antibody
cat.: 0806-7
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Glycophorin A aa 101-150 / 150.
Positive control: Human liver tissue lysate, 293, HepG2, human lung tissue, human placenta tissue, human spleen tissue, human kidney tissue, K562.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P02724 Human
Alternative names: AI853584 Blood group--MN locus CD_antigen=CD235a CD235a GLPA_HUMAN Glycophorin A (MNS blood group) Glycophorin A Glycophorin A, included Glycophorin-A GlycophorinA GPA GPErik GpMiIII GPSAT GYPA GYPA, included HGpMiIII HGpMiV HGpMiX HGpMiXI HGpSta(C) MN MN sialoglycoprotein MNS PAS-2 PAS2 Sialoglycoprotein alpha
Images
0806-7_1.jpg Fig1: Western blot analysis of Glycophorin A on human liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
0806-7_2.jpg Fig2: ICC staining Glycophorin A in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0806-7) at a dilution of 1/50 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
0806-7_3.jpg Fig3: ICC staining Glycophorin A in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0806-7) at a dilution of 1/50 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
0806-7_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Glycophorin A antibody (0806-7) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0806-7_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Glycophorin A antibody (0806-7) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0806-7_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glycophorin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0806-7) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
0806-7_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glycophorin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0806-7) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
0806-7_8.jpg Fig8: Flow cytometric analysis of Glycophorin A was done on K562 cells. The cells were fixed, permeabilized and stained with Glycophorin A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-Rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.