LDHB Rabbit Polyclonal Antibody
cat.: 0807-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LDH-B2 aa 285-334 / 334. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate, A549, rat kidney tissue, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:500-1:1,000 1:200 1:500-1:1,000 1:200-1:500 |
| Uniprot #: | SwissProt: P07195 Human | P16125 Mouse | P42123 Rat |
| Alternative names: | Epididymis secretory protein Li 281 HEL S 281 L lactate dehydrogenase B chain L-lactate dehydrogenase B chain Lactate dehydrogenase H chain LDH B LDH H LDH heart subunit LDH-B LDH-H LDHB LDHB_HUMAN LDHBD LDHH Renal carcinoma antigen NY REN 46 Renal carcinoma antigen NY-REN-46 TRG-5 TRG5 |
Images
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Fig1:
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (0807-1) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0807-1) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (0807-1) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Lactate Dehydrogenase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. 0807-1 was shown to specifically react with Lactate Dehydrogenase in Hela-si NT cells. Weakened band was observed when Hela-si Lactate Dehydrogenase sample was tested. Hela-si NT and Hela-si Lactate Dehydrogenase samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (0807-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling LDHB with Rabbit anti-LDHB antibody (0807-1) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LDHB antibody (0807-1) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-LDHB antibody (0807-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LDHB antibody (0807-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LDHB antibody (0807-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of A549 cells labeling LDHB. Cells were fixed and permeabilized. Then stained with the primary antibody (0807-1, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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