Insulin B Chain Rabbit Polyclonal Antibody
cat.: 0807-11
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: IHC-P, FC, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 12 kDa
Isotype: IgG
Immunogen: Full length human Insulin B Chain protein.
Positive control: Mouse pancreas tissue, human pancreas tissue, HepG2.
Subcellular location: Secreted.
Recommended Dilutions:
  IHC-P
  FC
  ELISA

1:200-1:2,000
1:50-1:100
1:5000
Uniprot #: SwissProt: P01308 Human
Alternative names: IDDM IDDM1 IDDM2 ILPR ins INS_HUMAN Insulin A chain Insulin B chain IRDN MODY11 Preproinsulin Proinsulin Proinsulin precursor
Images
0807-11_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0807-11_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0807-11_3.jpg Fig3: Flow cytometric analysis of Insulin B Chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (0807-11, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.