Insulin B Chain Rabbit Polyclonal Antibody
cat.: 0807-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, FC, ELISA, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Full length human Insulin B Chain protein. |
| Positive control: | Human pancreas tissue, mouse pancreas tissue, rat pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P FC ELISA IHC-Fr |
1:2,000-1:10,000 1:50-1:100 1:5,000 1:200 |
| Uniprot #: | SwissProt: P01308 Human | P01325 Mouse | P01322 Rat |
| Alternative names: | IDDM IDDM1 IDDM2 ILPR ins INS_HUMAN Insulin A chain Insulin B chain IRDN MODY11 Preproinsulin Proinsulin Proinsulin precursor |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Insulin B Chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (0807-11, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Insulin B Chain with Rabbit anti-Insulin B Chain antibody (0807-11) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (0807-11, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.