COX1/Cyclooxygenase 1 Rabbit Polyclonal Antibody
cat.: 0807-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 69 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human COX-1 aa 550-599.
Positive control: NIH/3T3 cell lysate, 293T cell lysate.
Subcellular location: Microsome membrane, Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P23219 Human | P22437 Mouse
Alternative names: COX 1 COX 3 COX-1 COX1 Cox3 Cyclooxygenase 1 Cyclooxygenase 3, included Cyclooxygenase-1 EC 1.14.99.1 Partial COX1 proteins, included PCOX1 PGG/HS PGH synthase 1 PGH1_HUMAN PGHS-1 PGHS1 PHS 1 PHS1 Prostaglandin G/H synthase 1 Prostaglandin H2 synthase 1 Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 1 PTGHS PTGS1
Images
0807-3_1.jpg Fig1: Western blot analysis of COX1/Cyclooxygenase 1 on NIH/3T3 cell lysate with Rabbit anti-COX1/Cyclooxygenase 1 antibody (0807-3) at 1/500 dilution.

Exposure time: 30 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0807-3, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 69 kDa
Observed band size: 69 kDa
0807-3_2.jpg Fig2: Western blot analysis of COX1/Cyclooxygenase 1 on 293T cell lysate with Rabbit anti-COX1/Cyclooxygenase 1 antibody (0807-3) at 1/500 dilution.

Exposure time: 30 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0807-3, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 69 kDa
Observed band size: 69 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.