| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human COX-1 aa 550-599. |
| Positive control: | NIH/3T3 cell lysate, 293T cell lysate. |
| Subcellular location: | Microsome membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P23219 Human | P22437 Mouse |
| Alternative names: | COX 1 COX 3 COX-1 COX1 Cox3 Cyclooxygenase 1 Cyclooxygenase 3, included Cyclooxygenase-1 EC 1.14.99.1 Partial COX1 proteins, included PCOX1 PGG/HS PGH synthase 1 PGH1_HUMAN PGHS-1 PGHS1 PHS 1 PHS1 Prostaglandin G/H synthase 1 Prostaglandin H2 synthase 1 Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 1 PTGHS PTGS1 |
|
Fig1:
Western blot analysis of COX1/Cyclooxygenase 1 on NIH/3T3 cell lysate with Rabbit anti-COX1/Cyclooxygenase 1 antibody (0807-3) at 1/500 dilution. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0807-3, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 69 kDa Observed band size: 69 kDa |
|
Fig2:
Western blot analysis of COX1/Cyclooxygenase 1 on 293T cell lysate with Rabbit anti-COX1/Cyclooxygenase 1 antibody (0807-3) at 1/500 dilution. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0807-3, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 69 kDa Observed band size: 69 kDa |