Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 69 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human COX-1 aa 550-599. |
Positive control: | NIH/3T3, 293T. |
Subcellular location: | Microsome membrane, Endoplasmic reticulum membrane. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: P23219 Human | P22437 Mouse |
Alternative names: | COX 1 COX 3 COX-1 COX1 Cox3 Cyclooxygenase 1 Cyclooxygenase 3, included Cyclooxygenase-1 EC 1.14.99.1 Partial COX1 proteins, included PCOX1 PGG/HS PGH synthase 1 PGH1_HUMAN PGHS-1 PGHS1 PHS 1 PHS1 Prostaglandin G/H synthase 1 Prostaglandin H2 synthase 1 Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 1 PTGHS PTGS1 |
Fig1:
Western blot analysis of COX1/Cyclooxygenase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate, untreated Lane 2: NIH/3T3 cell lysate, untreated |