LT-alpha (C-term) Rabbit Polyclonal Antibody
cat.: 0807-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LT-alpha aa 156-205 / 205. |
| Positive control: | Ramos cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Ramos. |
| Subcellular location: | Secreted, Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: P01374 Human | P09225 Mouse | Q06332 Rat |
| Alternative names: | LTalpha DIF LT alpha LT LT-alpha Lta Lymphotoxin alpha (TNF superfamily, member 1) Lymphotoxin alpha Lymphotoxin-alpha TNF B TNF superfamily member 1 TNF, lymphocyte-derived TNF-beta TNFB TNFB_HUMAN TNFbeta TNFSF1 TNLG1E Tumor necrosis factor beta tumor necrosis factor ligand 1E Tumor necrosis factor ligand superfamily member 1 |
Images
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Fig1:
Western blot analysis of LT-alpha (C-term) on different lysates with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Mouse skeletal muscle tissue lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 26 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0807-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of LT-alpha (C-term) on recombinant Human LTA protein (HA210742) with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/2,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0807-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Western blot analysis on LT-alpha(TNF-β) using anti- LT-alpha polyclonal antibody |
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Fig4:
Immunocytochemistry analysis of Ramos cells labeling LT-alpha (C-term) with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.