beta 2 Microglobulin Rabbit Polyclonal Antibody
cat.: 0808-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Beta-2 Microglobulin aa 21-70. |
| Positive control: | HL-60 cells. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:200 1:50 |
| Uniprot #: | SwissProt: P61769 Human |
| Alternative names: | B2M B2MG_HUMAN Beta 2 microglobin Beta 2 microglobulin Beta 2 microglobulin precursor Beta chain of mhc class 1 proteins Beta chain of MHC class I molecules Beta-2-microglobulin form pI 5.3 CDABP0092 Hdcma22p IMD43 |
Images
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Fig1:
Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (0808-2) at 1/5,000 dilution. Lane 1: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate (negative) Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 SH-SY5Y is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0808-2, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 14 kDa Observed band size: 14 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.