Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Mouse |
Applications: | WB, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 14 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse Ly-6G aa 1-50 / 134. |
Positive control: | Mouse spleen tissue lysate, Mouse lung tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB IHC-P |
1:5,00-1:2,000 1:100-1:500 |
Uniprot #: | SwissProt: P35461 Mouse |
Alternative names: | Gr-1 Gr1 Ly-6G.1 Ly6G Lymphocyte antigen 6 complex locus G Lymphocyte antigen 6G |
Fig1:
Western blot analysis of Ly-6G on different lysates with Rabbit anti-Ly-6G antibody (0809-11) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 25~30 kDa Exposure time: 7 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |