Ly6g Rabbit Polyclonal Antibody
cat.: 0809-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Ly6g aa 1-105. |
| Positive control: | Mouse spleen tissue lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P35461 Mouse |
| Alternative names: | Gr-1 Gr1 Ly-6G.1 Ly6G Lymphocyte antigen 6 complex locus G Lymphocyte antigen 6G |
Images
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Fig1:
Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (0809-11) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 25~30 kDa Exposure time: 7 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (0809-11) at 1/5,000 dilution. Lane 1: 293T transfected with empty control cell lysate (negative) Lane 2: 293T transfected with Ly6g cell lysate (positive) Lane 3: 293T transfected with Ly6f cell lysate (negative) Lane 4: 293T transfected with Ly6i cell lysate (negative) Lane 5: 293T transfected with Ly6c1 cell lysate (negative) Lane 6: 293T transfected with Ly6c2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.