Ly-6G Rabbit Polyclonal Antibody
cat.: 0809-11
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Ly-6G aa 1-50 / 134.
Positive control: Mouse spleen tissue lysate, Mouse lung tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:5,00-1:2,000
1:100-1:500
Uniprot #: SwissProt: P35461 Mouse
Alternative names: Gr-1 Gr1 Ly-6G.1 Ly6G Lymphocyte antigen 6 complex locus G Lymphocyte antigen 6G
Images
0809-11_1.jpg Fig1: Western blot analysis of Ly-6G on different lysates with Rabbit anti-Ly-6G antibody (0809-11) at 1/500 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse lung tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 14 kDa
Observed band size: 25~30 kDa

Exposure time: 7 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.