| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ApoE aa 19-58. |
| Positive control: | Human liver |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P02649 Human |
| Alternative names: | AD2 Apo-E APOE APOE_HUMAN APOEA Apolipoprotein E Apolipoprotein E3 ApolipoproteinE Apoprotein LDLCQ5 LPG |
|
Fig1:
Western blot analysis of Apolipoprotein E on Human serum lysate with Rabbit anti-Apolipoprotein E antibody (0809-6) at 1/2,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0809-6, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 36 kDa |