6X His tag Rabbit Polyclonal Antibody
cat.: 0812-1
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: WB, IP
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide immune sequence is HHHHHHC.
Positive control: C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein.
Recommended Dilutions:
  WB
  IP

1:500-1:2000
2-5 µg/ml.
Images
0812-1_1.jpg Fig1: Western blot analysis of 6X His tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0812-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein
0812-1_2.jpg Fig2: His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with 0812-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M0812-3 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

Lane 1: His Tag fusion protein lysate (input).
Lane 2: 0812-1 IP in His Tag fusion protein lysate.
Lane 3: R1207-2 IP in His Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of 0812-1 in His Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.