6X His tag Rabbit Polyclonal Antibody
cat.: 0812-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is HHHHHHC. |
| Positive control: | C-terminal His-tagged recombinant protein. |
| Recommended Dilutions:
WB IP |
1:500-1:2000 2-5 µg/ml. |
Images
|
Fig1:
Western blot analysis of 6X His tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0812-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C-terminal His-tagged recombinant protein Lane 2: N-terminal His-tagged recombinant protein |
|
Fig2:
His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with 0812-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M0812-3 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: His Tag fusion protein lysate (input). Lane 2: 0812-1 IP in His Tag fusion protein lysate. Lane 3: R1207-2 IP in His Tag fusion protein lysate. Lane 4: Rabbit IgG instead of 0812-1 in His Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.