| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PAI-3 aa 357-406 / 406. |
| Positive control: | Human liver tissue lysates. |
| Subcellular location: | Secreted, extracellular space. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P05154 Human |
| Alternative names: | Acrosomal serine protease inhibitor IPSP IPSP_HUMAN PAI 3 PAI-3 PAI3 PCI PCI-B PLANH 3 PLANH3 Plasma serine protease inhibitor Plasminogen activator inhibitor 3 Plasminogen activator inhibitor III PROCI Protein C inhibitor Serpin A5 Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5 Serpin peptidase inhibitor clade A member 5 Serpina5 |
|
Fig1:
Western blot analysis of PAI-3 / Serpin A5 on human liver tissue lysates with Rabbit anti-PAI-3 / Serpin A5 antibody (0812-5) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 46 kDa Observed band size: 42 kDa Exposure time: 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0812-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |