| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 52/80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Vitamin K-dependent protein C aa 412-461 / 461. |
| Positive control: | K562, human plasma |
| Subcellular location: | Secretory protein |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P04070 Human |
| Alternative names: | Activation peptide Anticoagulant protein C APC Autoprothrombin IIA Blood coagulation factor XIV EC 3.4.21.69 PC proC PROC_HUMAN PROC1 Protein C (inactivator of coagulation factors Va and VIIIa) THPH3 THPH4 Vitamin K dependent protein C |
|
Fig1:
Western blot analysis of Vitamin K-dependent protein C on different lysates with Rabbit anti-Vitamin K-dependent protein C antibody (0812-6) at 1/2,000 dilution. Lane 1:human plasma lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds ; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0812-6, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52/57 kDa Observed band size: 57 kDa |