ST2 Rabbit Polyclonal Antibody
cat.: 0902-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse IL1RL1 aa 13-62 / 567. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, Zebrafish tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:500-2,000 |
| Uniprot #: | SwissProt: Q01638 Human | P14719 Mouse |
| Alternative names: | DER-4 DER4 FIT 1 Growth stimulation expressed homolog of mouse growth stimulation-expressed Il1rl1 IL33R ILRL1_HUMAN Interleukin 1 receptor like 1 interleukin 1 receptor related protein Interleukin-1 receptor-like 1 Protein ST2 ST2 ST2L ST2V T1 T1 protein |
Images
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Fig1:
Western blot analysis of ST2 on different lysates with Rabbit anti-ST2 antibody (0902-2) at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0902-2, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa, 63 kDa |
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Fig2: Western blot analysis of ST2 on Zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0902-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.