IL-13Ra1 Rabbit Polyclonal Antibody
cat.: 0902-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IL13Ra1 aa 102-151 / 427. |
| Positive control: | D3, HepG2, Hela |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P78552 Human |
| Alternative names: | bB128O4.2.1 (interleukin 13 receptor, alpha 1) Cancer/testis antigen 19 CD213a1 CD213A1 Antigen CT 19 CT19 I13R1_HUMAN IL 13 receptor subunit alpha 1 IL 13R alpha 1 IL 13R subunit alpha 1 IL 13Ra IL 13RA1 IL-13 receptor subunit alpha-1 IL-13R subunit alpha-1 IL-13R-alpha-1 IL-13RA1 IL13 receptor alpha 1 chain IL13R IL13RA Il13ra1 Interleukin 13 receptor alpha 1 Interleukin 13 receptor alpha 1 chain interleukin 13 receptor subunit alpha 1 Interleukin-13 receptor subunit alpha-1 NR 4 NR4 |
Images
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Fig1:
All lanes: Western blot analysis of IL13RA1 with anti-IL13RA1 antibody (0902-4) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: IL13RA1 knockout Hela whole cell lysate. 0902-4 was shown to specifically react with IL13RA1 in Wild-type Hela cells. No band was observed when IL13RA1 knockout samples were tested. Wild-type and IL13RA1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-IL13RA1 antibody (0902-4, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
|
Fig2: Western blot analysis on D3 (A) and HepG2 (B) using anti IL13Ra1 polyclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.