Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 80 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N-terminal Human TMEM132B. |
Positive control: | Hela cell lysate, mouse brain tissue lysate. |
Subcellular location: | Cell membrane |
Recommended Dilutions:
WB |
1:500~1:1,000 |
Uniprot #: | SwissProt: Q14DG7 Human |
Alternative names: | KIAA1786 KIAA1906 transmembrane protein 132B |
Fig1:
Western blot analysis of TMEM132B on different lysates with Rabbit anti-TMEM132B antibody (0903-1) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 1 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |