TMEM132B Rabbit Polyclonal Antibody
cat.: 0903-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 119 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human TMEM132B. |
| Positive control: | HeLa cell lysate, SK-Br-3 cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q14DG7 Human Entrez Gene: 208151 Mouse | 304458 Rat |
| Alternative names: | KIAA1786 KIAA1906 transmembrane protein 132B |
Images
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Fig1:
Western blot analysis of TMEM132B on different lysates with Rabbit anti-TMEM132B antibody (0903-1) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 119 kDa Observed band size: 80/130 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-1) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.