DOPEY2 Rabbit Polyclonal Antibody
cat.: 0903-3
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 258kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DOPEY2 aa 1025-1074 / 2,298.
Positive control: Mouse heart, human heart, Siha, N2A, Mouse brain,
Subcellular location: Early endosome membrane, Golgi apparatus membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:500-1:1,000
1:50-1:200
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: Q9Y3R5 Human | Q3UHQ6 Mouse
Alternative names: Dipeptidase 2 Dipeptidase2 DPEP 2 MBD 2 MBD2
Images
0903-3_1.jpg Fig1: Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.
0903-3_2.png Fig2: ICC staining Dopey-2 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0903-3_3.png Fig3: ICC staining Dopey-2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
0903-3_4.png Fig4: Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin.
0903-3_5.jpg Fig5: Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin.
0903-3_6.jpg Fig6: Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.