DOPEY2 Rabbit Polyclonal Antibody
cat.: 0903-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 258kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DOPEY2 aa 1025-1074 / 2,298. |
| Positive control: | Mouse heart, human heart, Siha, N2A, Mouse brain, |
| Subcellular location: | Early endosome membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y3R5 Human | Q3UHQ6 Mouse |
| Alternative names: | Dipeptidase 2 Dipeptidase2 DPEP 2 MBD 2 MBD2 |
Images
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Fig1: Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution. |
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Fig2: ICC staining Dopey-2 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Dopey-2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin. |
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Fig5: Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin. |
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Fig6: Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.