| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TMEM132E aa 10-59 / 1,074. |
| Positive control: | HEK-293 (Human embryonic kidney cell) cell lysate, HEL (Human erythroleukemia cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB |
1:500-1:5,000 |
| Uniprot #: | SwissProt: Q6IEE7 Human | Q6IEE6 Mouse |
| Alternative names: | T132E_HUMAN TMEM132E Transmembrane protein 132E |
|
Fig1:
Western blot analysis of TMEM132E on different lysates with Rabbit anti-TMEM132E antibody (0903-4) at 1/5,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: HEL (Human erythroleukemia cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0903-4, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 116 kDa Observed band size: 150 kDa |