Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 154 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human TMEM2 aa 851-900 / 1,383. |
Positive control: | A549 cell lysate, PC-3M cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:5,000 |
Uniprot #: | SwissProt: Q9UHN6 Human | Q5FWI3 Mouse Entrez Gene: 309400 Rat |
Alternative names: | KIAA1412 TMEM 2 tmem2 TMEM2_HUMAN Transmembrane protein 2 |
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Fig1:
Western blot analysis of TMEM2 on different lysates with Rabbit anti-TMEM2 antibody (0903-5) at 1/5,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: PC-3M cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 154 kDa Observed band size: 200 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |