Meckelin Rabbit Polyclonal Antibody
cat.: 0903-7
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 112 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Meckelin aa 20-80.
Positive control: MCF7 cell lysates, Hela cell lysate, mouse kidney tissue, human placenta tissue, human breast cancer tissue, PANC-1.
Subcellular location: Cytoskeleton, Plasma membrane, Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q5HYA8 Human | Q8BR76 Mouse
Alternative names: JBTS6 Meckel syndrome type 3 protein MKS3 NPHP11 TMEM67 TNEM67 Transmembrane protein 67
Images
0903-7_1.jpg Fig1: Western blot analysis of Meckelin on different lysates with Rabbit anti-Meckelin antibody (0903-7) at 1/1,000 dilution.

Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate

Lysates/proteins at 10 µg/Lane.
Exposure time: 110s seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0903-7, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 112 kDa
Observed band size: 100 kDa
0903-7_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0903-7_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0903-7_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
0903-7_5.jpg Fig5: Flow cytometric analysis of Meckelin was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (0903-7, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.