FAT tumor suppressor homolog 4 Rabbit Polyclonal Antibody
cat.: 0905-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 543 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FAT tumor suppressor homolog 4 aa 2,264-2,313 / 4,981. |
| Positive control: | HepG2, Lovo. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IF-Cell |
1:500～1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q6V0I7 Human |
| Alternative names: | Cadherin family member 14 Cadherin related family member 11 CDHF14 CDHR11 FAT atypical cadherin 4 FAT J FAT tumor suppressor homolog 4 Fat-like cadherin protein FAT-J FAT4 FAT4_HUMAN FATJ hFat4 HKLLS2 Nbla00548 Protocadherin Fat 4 Putative protein product of Nbla00548 VMLDS2 |
Images
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Fig1: Western blot analysis on recombinant FAT4 using anti-FAT4 polyclonal antibody. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling FAT tumor suppressor homolog 4 with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of Lovo cells labeling FAT tumor suppressor homolog 4 with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FAT tumor suppressor homolog 4 antibody (0905-1) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.