Frizzled-5 Rabbit Polyclonal Antibody
cat.: 0905-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Frizzled-5 aa 143-192 / 585. |
| Positive control: | Human ES cell, A549, LOVO, HepG2. |
| Subcellular location: | Cell membrane.Golgi apparatus. Membrane. Synapse. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q13467 Human |
| Alternative names: | C2orf31 DKFZP434E2135 Frizzled 5 seven transmembrane spanning receptor Frizzled class receptor 5 Frizzled family receptor 5 Frizzled homolog 5 Frizzled-5 Frizzled5 Fz 5 Fz-5 Fz5 FZD 5 fzd5 FZD5_HUMAN FzE 5 FzE5 HFZ 5 hFz5 MGC129692 Seven transmembrane receptor frizzled 5 Uncharacterized protein C2orf31 Wnt receptor |
Images
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Fig1: ICC staining Frizzled-5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining Frizzled-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0905-3) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of Frizzled-5 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Frizzled-5 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution. |
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Fig4:
Western blot analysis of Frizzled-5 on different lysates with Rabbit anti-Frizzled-5 antibody (0905-3) at 1/1,000 dilution. Lane 1: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0905-3, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 64 kDa Observed band size: 70 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.