FAT1 Rabbit Polyclonal Antibody
cat.: 0905-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 506 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Protocadherin Fat 1 aa 238-287 / 4,588. |
| Positive control: | Recombinant protein |
| Subcellular location: | Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-5,000 1:100-1:400 |
| Uniprot #: | SwissProt: Q14517 Human |
| Alternative names: | Cadherin family member 7 Cadherin family member 7 precursor Cadherin ME5 Cadherin related tumor suppressor Cadherin related tumor suppressor homolog Cadherin related tumor suppressor homolog precursor Cadherin related tumor suppressor homolog precursor (FAT protein homolog) Cadherin-related tumor suppressor homolog CDHF 7 CDHF7 FAT 1 FAT tumor suppressor 1 FAT tumor suppressor homolog 1 FAT1 FAT1_HUMAN hFat 1 hFAT hFat1 Homolog of Drosophila FAT protein Homolog of Drosophila FAT protein precursor Homolog of Drosophila tumor suppressor FAT precursor ME 5 ME5 nuclear form Protein fat homolog |
Images
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Fig1:
Western blot analysis of FAT1 on recombinant Fat1 protein with Rabbit anti-FAT1 antibody (0905-4) at 1/5,000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0905-4) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human Stomach tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.