HA tag Rabbit Polyclonal Antibody
cat.: 0906-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is YPYDVPDYAC. |
| Positive control: | C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein. |
| Recommended Dilutions:
WB IP |
1:5,000-1:10,000 2-5 µg/ml. |
| Alternative names: | HA Tag |
Images
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Fig1:
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with 0906-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: ET1611-49 IP in HA Tag fusion protein lysate. Lane 3: 0906-1 IP in HA Tag fusion protein lysate. Lane 4: Rabbit IgG instead of 0906-1 in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
|
Fig2:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (0906-1) at 1/2,000 dilution. Lane 1: C-terminal HA-tagged recombinant protein Lane 2: N-terminal HA-tagged recombinant protein Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 0906-1, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.