Autoimmune regulator Rabbit Polyclonal Antibody
cat.: 0908-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size:59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Mouse Autoimmune regulator aa 503-552 / 552. |
| Positive control: | Transfected 293 cells, human pancreatic tissue, mouse pancreatic tissue. |
| Subcellular location: | Cell nuclear, cytoplasm |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:500 1:100-1:400 |
| Uniprot #: | SwissProt: Q9Z0E3 Mouse | O43918 Human |
| Alternative names: | AIRE AIRE_HUMAN AIRE1 APECED APECED protein APS1 APSI Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein Autoimmune regulator Autoimmune regulator protein PGA1 |
Images
|
Fig1: Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreatic tissue with Rabbit anti-Autoimmune regulator antibody (0908-2) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0908-2) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreatic tissue with Rabbit anti-Autoimmune regulator antibody (0908-2) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0908-2) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.