c-Myc Rabbit Polyclonal Antibody
cat.: 0912-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human C-myc aa 390-439. |
| Positive control: | Myc-tagged recombinant protein, A549 cell lysate, Raji cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P01106 Human |
| Alternative names: | AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Class E basic helix-loop-helix protein 39 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 MYCC Myelocytomatosis oncogene Niard Nird Oncogene Myc OTTHUMP00000158589 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) |
Images
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Fig1: Western blot analysis on myc-tagged recombinant protein (A) and A549 cell lysates (B). |
|
Fig2:
Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (0912-2) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0912-2) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.