HRP Conjugated DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
cat.: 0912-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is CDYKDDDDK. |
| Positive control: | DYKDDDDK Tag recombinant protein |
| Recommended Dilutions:
WB |
1:10,000 |
| Alternative names: | DDDDK epitope tag DDDK ddk DYKDDDDK DYKDDDDK epitope tag DYKDDDDK tag ECS epitope tag ECS tag Enterokinase Cleavage Site epitope tag Enterokinase Cleavage Site tag FLAG FLAG tag |
Images
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Fig1:
Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Rabbit anti-DYKDDDDK Tag (FLAG) antibody (0912-3) at 1/10,000 dilution. Lane 1: HeLa transfected with empty control cell lysate (20 µg/Lane) Lane 2: HeLa transfected with FLAG-tagged Otx1 (C-terminal) cell lysate (10 µg/Lane) Lane 3: 293T transfected with empty control cell lysate (20 µg/Lane) Lane 4: 293T transfected with FLAG-tagged MYRF (N-terminal) cell lysate (40 µg/Lane) Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0912-3) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.