CD147 Rabbit Polyclonal Antibody
cat.: 1003-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to mouse CD147 aa 121-170 / 389 mouse. |
| Positive control: | Mouse embryo, PC-12 cell lysates, NIH/3T3, F9. |
| Subcellular location: | Cell membrane, Photoreceptor inner segment, Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50 1ug/mL |
| Uniprot #: | SwissProt: P18572 Mouse | P26453 Rat |
| Alternative names: | 5A11 antigen 5F7 BASI_HUMAN Basigin (Ok blood group) Basigin Blood brain barrier HT7 antigen Bsg CD 147 CD147 CD147 antigen Collagenase stimulatory factor EMMPRIN Extracellular matrix metalloproteinase inducer Leukocyte activation antigen M6 M 6 M6 M6 leukocyte activation antigen Neurothelin OK OK blood group OK blood group antigen TCSF Tumor cell derived collagenase stimulatory factor Tumor cell-derived collagenase stimulatory factor |
Images
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Fig1: Western blot analysis on mouse embryo using anti-CD147 polyclonal antibody. |
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Fig2:
Western blot analysis of CD147 on PC-12 cell lysates with Rabbit anti-CD147 antibody (1003-10) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling CD147 with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD147 antibody (1003-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of F9 cells labeling CD147. Cells were fixed and permeabilized. Then stained with the primary antibody (1003-10, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.