GST Rabbit Polyclonal Antibody
cat.: 1003-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within GST protein. |
| Positive control: | GST fusion-protein |
| Recommended Dilutions:
WB ELISA IP |
1:10,000-1:100,000 1:10,000 1-2μg/sample |
| Alternative names: | Glutathione S Transferase Glutathione S-transferase class-mu 26 kDa isozyme GST Sj26 antigen SjGST |
Images
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Fig1:
GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with 1003-8 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 1003-8 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input) Lane 2: Rabbit IgG instead of 1003-8 in 293T transfected with GST-tagged Histone H3.1 cell lysate Lane 3: 1003-8 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds |
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Fig2:
Immunocytochemistry analysis of 293T cells transfected with or without GST labeling GST with Rabbit anti-GST antibody (1003-8) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (1003-8) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (1003-8) at 1/80,000 dilution. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-8) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.