CD271 Rabbit Polyclonal Antibody
cat.: 1003-9
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Mouse CD271 aa 91-140 / 427 mouse.
Positive control: F9 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, L6.
Subcellular location: Cell membrane, Perikaryon, Cell projection, growth cone, Cell projection, dendritic spine.
Recommended Dilutions:

Uniprot #: SwissProt: Q9Z0W1 Mouse | P07174 Rat
Alternative names: CD271 CD271 antigen Gp80 LNGFR Gp80-LNGFR Low affinity nerve growth factor receptor Low affinity neurotrophin receptor p75NTR Low-affinity nerve growth factor receptor Nerve growth factor receptor Nerve growth factor receptor TNFR superfamily member 16 NGF receptor Ngfr p75 ICD p75 Neurotrophin receptor p75 NTR p75(NTR) p75NTR TNFR Superfamily Member 16 TNFRSF16 TNR16_HUMAN Tumor necrosis factor receptor superfamily member 16
1003-9_1.jpg Fig1: Western blot analysis of CD271 on different lysates with Rabbit anti-CD271 antibody (1003-9) at 1/1,000 dilution.

Lane 1: F9 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: RAW264.7 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
1003-9_2.jpg Fig2: Immunocytochemistry analysis of L6 cells labeling CD271 with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.