HRP Conjugated GST Rabbit Polyclonal Antibody
cat.: 1006-3
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within GST protein. |
| Recommended Dilutions:
WB ELISA |
1:5,000-1:10,000 1:10,000 |
| Alternative names: | Glutathione S Transferase Glutathione S-transferase class-mu 26 kDa isozyme GST Sj26 antigen SjGST |
Images
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Fig1:
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (1006-3) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with GST cell lysate Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1006-3) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig2: Indirect ELISA analysis of GST was performed by coating wells of a 96-well plate with 50 µL per well of GST diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of GST polyclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.