Amyloid Beta A4 Precursor (APP) Rabbit Polyclonal Antibody
cat.: 1007-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 87 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Amyloid Beta.
Positive control: Hela cell lysate, HUVEC cell lysate, A549 cell lysate, D3.
Subcellular location: Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: P05067 Human
Alternative names: A4 amyloid protein A4_HUMAN AAA ABETA ABPP AD1 AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease amyloid protein Amyloid beta (A4) precursor protein Amyloid beta A4 protein Amyloid beta protein Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 Amyloid precursor protein APP APPI Beta amyloid peptide beta-amyloid peptide Beta-amyloid precursor protein Beta-APP40 Beta-APP42 C31 Cerebral vascular amyloid peptide CTFgamma CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) peptidase nexin II peptidase nexin-II PN 2 PN II PN-II PN2 PreA4 Protease nexin II Protease nexin-II S-APP-alpha S-APP-beta sAPP
Images
1007-5_1.jpg Fig1: Western blot analysis of Amyloid Beta A4 Precursor (APP) on different lysates with Rabbit anti-Amyloid Beta A4 Precursor (APP) antibody (1007-5) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: HUVEC cell lysate
Lane 3: A549 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1007-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
1007-5_2.jpg Fig2: ICC on D3.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.