| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Amyloid Beta. |
| Positive control: | Hela cell lysate, HUVEC cell lysate, A549 cell lysate, D3. |
| Subcellular location: | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P05067 Human |
| Alternative names: | A4 amyloid protein A4_HUMAN AAA ABETA ABPP AD1 AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease amyloid protein Amyloid beta (A4) precursor protein Amyloid beta A4 protein Amyloid beta protein Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 Amyloid precursor protein APP APPI Beta amyloid peptide beta-amyloid peptide Beta-amyloid precursor protein Beta-APP40 Beta-APP42 C31 Cerebral vascular amyloid peptide CTFgamma CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) peptidase nexin II peptidase nexin-II PN 2 PN II PN-II PN2 PreA4 Protease nexin II Protease nexin-II S-APP-alpha S-APP-beta sAPP |
|
Fig1: ICC on D3. |
|
Fig2:
Western blot analysis of Amyloid Beta A4 Precursor (APP) on different lysates with Rabbit anti-Amyloid Beta A4 Precursor (APP) antibody (1007-5) at 1/1,000 dilution. Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1007-5, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted MW: 87 kDa Observed MW: 105 kDa |